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version 2 microarray hybridization buffer  (Amersham Pharmacia Biotech Ltd)

 
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    Amersham Pharmacia Biotech Ltd version 2 microarray hybridization buffer
    Version 2 Microarray Hybridization Buffer, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/version+2+microarray+hybridization+buffer/version+2+microarray+hybridization+buffer/pm17470200-44-14-19
    Average 90 stars, based on 1 article reviews
    version 2 microarray hybridization buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Microarray:

    Article Title: Transcriptional program induced by factor VIIa-tissue factor, PAR1 and PAR2 in MDA-MB-231 cells.
    Article Snippet: Gaps II slides (Corning B.V, Schiphol-Rijk, The Netherlands) were spotted with polymerase chain reaction (PCR) products of approximately 8500 human genes (Unigene Human Cloneset Version 2.0) (Incyte Genomics, CA, USA) and prehybridized for 1.5 h in 2 · SSPE buffer (300 mM NaCl, 20 mM NaH2PO4, 2 mM EDTA, pH 7.4) with 0.1% sodium dodecylsulfate (SDS). .. Next, 1 lg polyA75 and 15 pmol Cy3- and Cy5-labeled cDNAs were mixed in Version 2 microarray hybridization buffer (Amersham Pharmacia Biotech, Piscataway, NJ, USA) and formamide (1:1, vol/vol) in a total volume of 40 lL, denatured at 95 C for 3 min and hybridized in a ISO20 microarray hybridization incubator (Grant Instruments, Cambridge, UK) at 42 C for 16 h. Slides were subsequently washed at 55 C in 1 · sodium chloride/sodium citrate buffer (SSC) (150 mM NaCl, 15 mM sodium citrate, pH 7.0) containing 0.2% SDS for 10 min, then twice in 0.1 · SSC, 0.2% SDS for 10 min, and in 0.1 · SSC for 1 min. .. Finally, slides were dried and scanned in an Axon4000B (Axon Instruments, Union City, CA, USA).

    Hybridization:

    Article Title: Transcriptional program induced by factor VIIa-tissue factor, PAR1 and PAR2 in MDA-MB-231 cells.
    Article Snippet: Gaps II slides (Corning B.V, Schiphol-Rijk, The Netherlands) were spotted with polymerase chain reaction (PCR) products of approximately 8500 human genes (Unigene Human Cloneset Version 2.0) (Incyte Genomics, CA, USA) and prehybridized for 1.5 h in 2 · SSPE buffer (300 mM NaCl, 20 mM NaH2PO4, 2 mM EDTA, pH 7.4) with 0.1% sodium dodecylsulfate (SDS). .. Next, 1 lg polyA75 and 15 pmol Cy3- and Cy5-labeled cDNAs were mixed in Version 2 microarray hybridization buffer (Amersham Pharmacia Biotech, Piscataway, NJ, USA) and formamide (1:1, vol/vol) in a total volume of 40 lL, denatured at 95 C for 3 min and hybridized in a ISO20 microarray hybridization incubator (Grant Instruments, Cambridge, UK) at 42 C for 16 h. Slides were subsequently washed at 55 C in 1 · sodium chloride/sodium citrate buffer (SSC) (150 mM NaCl, 15 mM sodium citrate, pH 7.0) containing 0.2% SDS for 10 min, then twice in 0.1 · SSC, 0.2% SDS for 10 min, and in 0.1 · SSC for 1 min. .. Finally, slides were dried and scanned in an Axon4000B (Axon Instruments, Union City, CA, USA).



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    microarray hybridization buffer version 2  (Amersham Life Sciences Inc)
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    Amersham Life Sciences Inc microarray hybridization buffer version 2
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    https://www.bioz.com/product/version+2+microarray+hybridization+buffer/microarray+hybridization+buffer/pm21130852-95-17-23
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    version 2 microarray hybridization buffer  (Amersham Pharmacia Biotech Ltd)
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    Amersham Pharmacia Biotech Ltd version 2 microarray hybridization buffer
    Version 2 Microarray Hybridization Buffer, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/version+2+microarray+hybridization+buffer/version+2+microarray+hybridization+buffer/pm17470200-44-14-19
    Average 90 stars, based on 1 article reviews
    version 2 microarray hybridization buffer - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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